作者：Guodong Rao,
Jinkai Sui, Yanfei Zeng, Caiyun He, Aiguo Duan, Jianguo Zhang*
期刊：Plos One
时间：2014年10月2日
卷.期.页码：DOI: 10.1371/journal.pone.0109122
摘要：Salix
matsudana Koidz. is a deciduous, rapidly growing,
and drought resistant tree and is one of the most widely distributed and
commonly cultivated willow species in China. Currently little transcriptomic
and small RNAomic data are available to reveal the genes involve in the stress
resistant in S. matsudana. Here, we report the RNA-seq analysis results of both transcriptome and
small RNAome data using Illumina deep sequencing of shoot tips from two willow
variants(Salix. matsudana and Salix matsudana Koidz. cultivar ‘Tortuosa’). De novo gene assembly was
used to generate the consensus transcriptome and small RNAome, which contained
106,403 unique transcripts with an average length of 944 bp and a total length
of 100.45 MB, and 166 known miRNAs representing 35 miRNA families. Comparison
of transcriptomes and small RNAomes combined with quantitative real-time PCR
from the two Salix libraries revealed
a total of 292 different expressed genes(DEGs) and 36 different expressed
miRNAs (DEMs). Among the DEGs and DEMs, 196 genes and 24 miRNAs were up
regulated, 96 genes and 12 miRNA were down regulated in S.
matsudana. Functional analysis of DEGs and miRNA
targets showed that many genes were involved in stress resistance in S.
matsudana. Our global gene expression profiling
presents a comprehensive view of the transcriptome and small RNAome which
provide valuable information and sequence resources for uncovering the stress
response genes in S. matsudana. Moreover the transcriptome and small RNAome data provide a basis for
future study of genetic resistance in Salix.
全文链接：http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0109122）