作者：Qingfen Li, Shougong Zhang and Junhui Wang*
期刊：Physiologia
Plantarum
影响因子：3.262
时间：2014年9月9日
卷.期.页码：DOI: 10.1111/ppl.12276
摘要：
The cytokinin 6-benzylaminopurine (6-BAP) influences the embryogenic capacity
of the tissues of Picea balfouriana during long subculture (after three
months). Tissues that proliferate in 3.6 μM and 5 μM 6-BAP exhibit the highest
and lowest embryogenic capacity, respectively, generating 113±6 and 23±3
mature embryos per 100?mg of tissue. In this study, a comparative
transcriptomic and proteomic approach was applied to characterize the genes and
proteins that are differentially expressed among tissues under the influence of
different levels of 6-BAP. A total of 51375 unigenes and 2617 proteins were
obtained after quality filtering. There were 2770 transcripts for proteins
found among these unigenes. Gene Ontology (GO) analysis of the differentially
expressed unigenes and proteins showed that they were involved in cell and
binding activity and were enriched in ribosome and glutathione metabolism
pathways. Ribosomal proteins, glutathione S-transferase proteins, germin-like
proteins and calmodulin-independent protein kinases were up-expressed in the
embryogenic tissues with the highest embryogenic ability (treated with 3.6 μM
6-BAP), which was validated via qRT-PCR analysis, and these proteins might
serve as molecular markers of embryogenic ability. Data are available via SRA
and ProteomeXchange with identifier SRP042246 and PXD001022, respectively.
全文链接：http://onlinelibrary.wiley.com/doi/10.1111/ppl.12276/abstract