作者：Danlong Jing, Yan Xia, Faju
Chen, Zhi Wang, Shougong Zhang, Junhui Wang*.
期刊：Plant science
发表时间：2015年
卷.期.页码：231: 40–51.
摘要：PISTILLATA (PI) plays crucial roles in Arabidopsis flower development by specifying
petal and stamen identities. To investigate the molecular mechanisms underlying
organ development of woody angiosperm in Catalpa,
we isolated and identified a PI
homologue, referred to as CabuPI (C. bungei PISTILLATA), from two genetically
cognate C. bungei (Bignoniaceae)
bearing single and double flowers. Sequence and phylogenetic analyses revealed
that the gene is closest related to the eudicot PI homologues. Moreover, a highly conserved PI-motif is found in the C-terminal regions of CabuPI. Semi-quantitative and quantitative rea ltime PCR analyses
showed that the expression of CabuPI
was restricted to petals and stamens. However, CabuPI expression in the petals and stamens persisted throughout
all floral development stages, but the expression levels were different. In 35S::CabuPI
transgenic homozygous pi-1 mutant
Arabidopsis, the second and the third whorl floral organs produced normal
petals and a different number of stamens, respectively. Furthermore, ectopic
expression of the CabuPI in
transgenic wild-type or heterozygote pi-1
mutant Arabidopsis caused the first
whorl sepal partially converted into a petal-like structure. These results
clearly reveal the functional conservation of PI homologues between C.
bungei and Arabidopsis.