Genetic diversity and population structure of Taxus cuspidata in the Changbai Mountains as assessed by chloroplast DNA sequences and microsatellite markers

作   者：程蓓蓓,郑勇奇,孙启武
期刊名称：Biochemical Systematics and Ecology
影响因子：0.967
卷 期 号：63
页     码：157-164
关键词：Taxus cuspidata; Changbai Mountains; genetic diversity; population structure; geographic pattern
论文摘要：
Abstract:
Taxus cuspidata in the Changbai Mountains of China, a tree species with high economic value because of its anticancer properties, is experiencing severe reduction in populations across its range in China. We examined one chloroplast DNA (cpDNA) region (petA-psbE) and 9 nuclear simple sequence repeats (SSRs) loci variations among seven populations to investigate the levels of genetic diversity and population structure. A moderate level of haplotype diversity (HT = 0.625), low nuclear microsatellite diversity (HE = 0.261 ± 0.028), significant genetic differences (FST = 0.065) and substantial gene flow (Nm = 2.806) were observed. Most of the total genetic variation was partitioned within the population (87.8 % and 94 % for cpDNA and SSR, respectively). Our haplotype identification permutation tests revealed that GST > NST, indicating an absence of phylogeographic structure in T. cuspidata. Neither STRUCTURE nor UPGMA analyses showed any geographic pattern in T. cuspidata populations. By comparatively analyzing the genetic diversity and survival situation of T. cuspidata, our results provide a theoretical foundation for the resource protection, utilization cultivation and breeding of this valuable plant.