Comparative proteomic analysis of mature pollen in triploid and diploid Populus deltoids

作   者：章小铃, 张进, 郭英华, 孙佩, 贾会霞, 樊玮, 卢孟柱, 胡建军*
期刊名称：International journal of molecular sciences
影响因子：3.257
卷 期 号：17
页     码：1475
关键词：
论文摘要：
Ploidy affects plant growth vigor and cell size, but the relative effects of pollen fertility and
allergenicity between triploid and diploid have not been systematically examined. Here we performed
comparative analyses of fertility, proteome, and abundances of putative allergenic proteins of pollen
in triploid poplar ‘ZhongHuai1’ (‘ZH1’, triploid) and ‘ZhongHuai2’ (‘ZH2’, diploid) generated
from the same parents. The mature pollen was sterile in triploid poplar ‘ZH1’. By applying
two-dimensional gel electrophoresis (2-DE), a total of 72 differentially expressed protein spots (DEPs)
were detected in triploid poplar pollen. Among them, 24 upregulated and 43 downregulated proteins
were identified in triploid poplar pollen using matrix-assisted laser desorption/ionisation coupled
with time of-flight tandem mass spectrometer analysis (MALDI-TOF/TOF MS/MS). The main
functions of these DEPs were related with “S-adenosylmethionine metabolism”, “actin cytoskeleton
organization”, or “translational elongation”. The infertility of triploid poplar pollen might be related
to its abnormal cytoskeletal system. In addition, the abundances of previously identified 28 putative
allergenic proteins were compared among three poplar varieties (‘ZH1’, ‘ZH2’, and ‘2KEN8‘). Most
putative allergenic proteins were downregulated in triploid poplar pollen. This work provides an
insight into understanding the protein regulation mechanism of pollen infertility and low allergenicity
in triploid poplar, and gives a clue to improving poplar polyploidy breeding and decreasing the
pollen allergenicity.