Competing Endogenous RNA Networks Underlying Anatomical and Physiological Characteristics of Poplar Wood in Acclimation to Low Nitrogen Availability

作   者：逯岩,邓澍荣,李卓蓉,吴江婷,刘奇峰,刘文哲,于文剑,张玉红,石文广,周婧,李红,Andrea Polle,罗志斌
期刊名称：Plant & Cell Physiology
影响因子：3.929
卷 期 号：60
页     码：2478–2495
关键词：Amino acids Carbohydrates Metabolomics
论文摘要：
Although poplar plantations are often established on nitrogen (N)-poor soil, the physiological and molecular mechanisms underlying wood properties of poplars in acclimation to
low N availability remain largely unknown. To investigate
wood properties of poplars in acclimation to low N,
Populus canescens saplings were exposed to either 50
(low N) or 500 (normal N) mM NH4NO3 for 2 months. Low
N resulted in decreased xylem width and cell layers of the
xylem (the number of cells counted along the ray parenchyma on the stem cross section), narrower lumina of vessels
and fibers, greater thickness of double fiber walls (the walls
between two adjacent fiber cells), more hemicellulose and
lignin deposition, and reduced cellulose accumulation in
poplar wood. Consistently, concentrations of gibberellins
involved in cell size determination and the abundance of
various metabolites including amino acids, carbohydrates
and precursors for cell wall biosynthesis were decreased in
low N-supplied wood. In line with these anatomical and
physiological changes, a number of mRNAs, long noncoding
RNAs (lncRNAs) and microRNAs (miRNAs) were significantly differentially expressed. Competing endogenous
RNA regulatory networks were identified in the wood of
low N-treated poplars. Overall, these results indicate that
miRNAs–lncRNAs–mRNAs networks are involved in regulating wood properties and physiological processes of poplars
in acclimation to low N availability