Proteomic analysis and identification of possible allergenic proteins in mature pollen of Populus tomentosa

作   者：王留强，章小玲，张进，范伟，卢孟柱，胡建军
期刊名称：International Journal of Molecular Sciences
影响因子：3.687
卷 期 号：19(1)
页     码：250
关键词： allergen;MALDI-TOF/TOFMS/MS; pollen; Populus tomentosa; two-dimensional gel electrophoresis
论文摘要：
Pollen grains from Populus tomentosa, a widely cultivated tree in northern area of China,
are considered to be an important aeroallergen causing severe allergic diseases. To gain insight
into their allergenic components, mature Populus tomentosa pollen proteins were analyzed by
two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization-time
of flight mass spectrometry (MALDI-TOF/TOF MS). A total of 412 spots from mature pollen were
resolved on pH 4–7 immobilized pH gradient (IPG) strips and 159 distinct proteins were identified
from 242 spots analyzed. The identified proteins were categorized based on their functional role in
the pollen, which included proteins involved in energy regulation, protein fate, protein synthesis
and processing, metabolism, defense/stress responses, development and other functional categories.
Moreover, among the identified proteins, 27 proteins were identified as putative allergens using the
Structural Database of Allergenic Proteins (SDAP) tool and Allergen Online. The expression patterns
of these putative allergen genes indicate that several of these genes are highly expressed in pollen.
The identified putative allergens have the potential to improve specific diagnosis and can be used to
develop vaccines for immunotherapy against poplar pollen allergy.