Whole-genome re-sequencing reveals molecular mechanisms of biomass changes in 11-year-old Bt transgenic poplar

作   者：张进，詹天一，贾会霞，吴立栓，卢孟柱，胡建军
期刊名称：TREES-STRUCTURE AND FUNCTION
影响因子：1.782
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关键词：Transgenic poplar · Bt · Wood characteristic · Whole-genome re-sequencing · InDels · Protein–protein interaction network
论文摘要：
Poplar is a fast-growing tree that relieves the wood demand in China. Previously, we found growth and wood characteristics
differed in some lines of insect-resistant Bt transgenic poplar. Here, we selected five Bt transgenic lines (#12, #153,
#172, #208 and #209), one untransformed control (CK2), one comparable control (CK3), and one native cultivar (JY, Populus
× euramericana ‘Robusta’) in Xinjiang, China, for further study. Based on breast diameter, tree height, chemical content,
and fibre morphology analyses, these transgenic lines showed fast growth and high-lignin and low-cellulose contents in the
cell wall. In addition, genome-wide insertions/deletions (InDels) were compared between two transgenic lines (#12 and #153)
and the untransformed control via whole-genome re-sequencing. GO enrichment and protein–protein interaction networks
indicated the genes affected by differential InDels were related to programmed cell death and defence responses. This study
provides new insight into the molecular mechanisms underlying changes in biomass characteristics in Bt transgenic poplar.