Mesorhizobium ephedrae sp. nov. isolated from the roots of Ephedra przewalskii in Kumtag desert.

作   者：Lei Liu, Li-xiong Liang, Xiao-xia Zhang, Lu-bin Li, Qi-wu Sun.
期刊名称：International Journal of Systematic and Evolutionary Microbiology
影响因子：1.931
卷 期 号：68
页     码：3615-3620
关键词：Mesorhizobium; Mesorhizobium ephedrae; Ephedra przewalskii; endophytic bacterium; Kumtag desert.
论文摘要：
Strain 6GN-30T, a Gram-stain-negative, aerobic, non-spore-forming, rod-shaped, motile bacterium was isolated from Ephedra
sinica roots in the Kumtag Desert. On the basis of the 16S rRNA gene sequence, the isolate represented a member of the
genus Mesorhizobium of the family Phyllobacteriaceae. The results of a phylogenetic analysis indicated that 6GN-30T was
phylogenetically related to Mesorhizobium soli NHI-8T. Strain 6GN-30T grew at a salinity of 0–1.0% (w/v) NaCl (with optimum
growth in the absence of NaCl), pH 6.0–9.0 (optimum 7.0–8.0) and 15–45 C. The major cellular fatty acids were summed
feature 8 (C18 : 1!7c/C18 : 1!6c), C19 : 0cyclo !8c, iso-C17 : 0, C18 : 0, and C16 : 0. The draft genome of 6GN-30T was 6.11 Mb long,
with a DNA G+C content of 66.4 mol%. The average nucleotide identity to M. soli NHI-8T was 84.32 %. The strain contained
diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine,aminophospholipids and
phospholipids. The chemotaxonomic, phylogenetic and phenotypic data indicate that 6GN-30T represents a novel species of
the genus Mesorhizobium for which the name Mesorhizobium ephedrae sp. nov. is proposed. The type strain is 6GN-30T
(=ACCC 60073T=KCTC 62410T).