Zobellella endophytica sp. nov., isolated from the roots of Phragmites communis in the Kumtag Desert

作   者：Zheng Song, Qi–wu Sun, Li–xiong Liang, Xiao–xia Zhang, Lu–bin Li, Lei Liu.
期刊名称：International Journal of Systematic and Evolutionary Microbiology
影响因子：1.931
卷 期 号：
页     码：
关键词：Zobellella endophytica; Phragmites communis; endophytic bacterium; Kumtag Desert.
论文摘要：
A Gram-negative, aerobic, non-spore-forming, rod-shaped, motile bacterium, named strain 59N8T, was isolated from
Phragmites communis roots in the Kumtag Desert. Phylogenetic analysis based on the 16S rRNA gene sequence showed that
the isolate belongs to the genus Zobellella within the family Aeromonadaceae. The analysis showed that strain 59N8T was
most closely related to Zobellella taiwanensis ZT1T. The average nucleotide identity value with Zobellella taiwanensis ZT1T was
88.2 %, and the digital DNA–DNA hybridization value was 29.7±2.4 %, which was calculated using the Genome-to-Genome
Distance Calculator. The G+C content of strain 59N8T was 62.8 mol%. Strain 59N8T grew at 0–5% (w/v) NaCl (optimum, 0–
4 %), pH 6.0–9.0 (optimum, 7.0–8.0) and at 10–45 C. The major cellular fatty acids were summed feature 8 (C18 : 1!7c/C18 : 1
!6c), summed feature 3 (C16 : 1!7c/C16 : 1!6c) and C16 : 0. The major polar lipids in strain 59N8T were
phosphatidylethanolamine and phosphatidylglycerol. Based on the chemotaxonomic, phylogenetic and phenotypic data, strain
59N8T represents a novel species in the genus Zobellella, for which the name Zobellella endophytica sp. nov. is proposed. The
type strain is 59N8T (=ACCC 60074T=KCTC 62456T).