Sphingobacterium haloxyli sp. nov., an endophytic bacterium isolated from Haloxylon ammodendron stems in Kumtag desert.

作   者：Lei Liu, Nan Hui, Li-xiong Liang, Xiao-xia Zhang, Lu-bin Li, Qi-wu Sun.
期刊名称：International Journal of Systematic and Evolutionary Microbiology
影响因子：1.931
卷 期 号：68
页     码：3279-3284
关键词：Sphingobacterium; Sphingobacterium haloxyli; Haloxylon ammodendron; endophytic bacterium; Kumtag desert.
论文摘要：
A Gram-stain-negative, non-motile, aerobic, non-spore-forming, rod-shaped, bacterial strain, designated 5JN-11T, was
isolated from Haloxylonammodendron stems in Kumtag desert, Xinjiang province, China. Strain 5JN-11T grew at salinities of
0–6% (w/v; optimum 0–2 %), a pH of 7.0–9.0 (pH 7.0–8.0) and temperatures of 20–42 C (28–30 C). Based on 16S rRNA gene
sequences, the strain was designated a member of the genus Sphingobacterium and the phylogenetic analysis showed that
strain 5JN-11T shared the highest similarity to Sphingobacterium gobiense H7T, followed by Sphingobacterium chuzhouense
DH-5T and Sphingobacterium arenae H-12T. The unfinished draft genome of strain 5JN-11T was 4.69 Mb. The
G+C content of strain 5JN-11T was 42.8 mol%. The average nucleotide identity to S. gobiense H7T was 90.5 %. The respiratory
quinone was MK-7, and the major polar lipids were phosphatidylethanolamine and phosphoglycolipid. The predominant
cellular fatty acids were summed feature 3 (C16 : 1!7c and/or C16 : 1!6c), iso-C15 : 0 and iso-C17 : 0 3-OH. On the basis of
phenotypic, genotypic and phylogenetic evidence, strain 5JN-11T represents a novel species in the genus Sphingobacterium,
for which the name Sphingobacterium haloxyli sp. nov. is proposed. The type strain is 5JN-11T (=ACCC 60072T=KCTC 62457T).