Genome-Wide Identification and Analysis of MicroRNAs Involved in Witches'-Broom Phytoplasma Response in Ziziphus jujuba

作   者：邵芬娟，张倩，刘洪伟，卢善发，邱德有
期刊名称：PLOSONE
影响因子：2.806
卷 期 号：11(11)
页     码：
关键词：miRNA,丛枝病，枣树
论文摘要：
MicroRNAs (miRNAs) play an important role in responding to biotic and abiotic stresses in
plants. Jujube witches’-broom a phytoplasma disease of Ziziphus jujuba is prevalent in
China and is a serious problem to the industry. However, the molecular mechanism of the
disease is poorly understood. In this study, genome-wide identification and analysis of
microRNAs in response to witches’-broom was performed. A total of 85 conserved miRNA
unique sequences belonging to 32 miRNA families and 24 novel miRNA unique sequences,
including their complementary miRNA* strands were identified from small RNA libraries
derived from a uninfected and witches’-broom infected Z. jujuba plant. Differentially
expressed miRNAs associated with Jujube witches’-broom disease were investigated
between the two libraries, and 12 up-regulated miRNAs and 10 down- regulated miRNAs
identified with more than 2 fold changes. Additionally, 40 target genes of 85 conserved miRNAs
and 49 target genes of 24 novel miRNAs were predicted and their putative functions
assigned. Using the modified 5’-RACE method, we confirmed that SPL and MYB were
cleaved by miR156 and miR159, respectively. Our results provide insight into the molecular
mechanisms of witches’-broom disease in Z. jujuba.