Metabolic responses of poplar to Apripona germari (Hope) as revealed by metabolite profiling

作   者：王丽娟†, 曲良建†, 张丽伟, 胡建军, 唐芳, 卢孟柱*
期刊名称：International journal of molecular sciences
影响因子：3.257
卷 期 号：17
页     码：6
关键词：
论文摘要：
Plants have developed biochemical responses to adapt to biotic stress. To characterize the
resistance mechanisms in poplar tree against Apripona germari, comprehensive metabolomic changes
of poplar bark and xylem in response to A. germari infection were examined by gas chromatography
time-of-flight mass spectrometry (GC–TOF/MS). It was found that, four days after feeding (stage I),
A. germari infection brought about changes in various metabolites, such as phenolics, amino acids
and sugars in both bark and xylem. Quinic acid, epicatechin, epigallocatechin and salicin might
play a role in resistance response in bark, while coniferyl alcohol, ferulic acid and salicin contribute
resistance in xylem. At feeding stages II when the larvae fed for more than one month, fewer defensive
metabolites were induced, but levels of many intermediates of glycolysis and the tricarboxylic acid
(TCA) cycle were reduced, especially in xylem. These results suggested that the defense strategies
against A. germari might depend mainly on the early defense responses in poplar. In addition, it
was found that bark and xylem in infected trees accumulated higher levels of salicylic acid and
4-aminobutyric acid, respectively, these tissues displaying a direct and systemic reaction against
A. germari. However, the actual role of the two metabolites in A. germari-induced defense in poplar
requires further investigation.