Acidobacteria Community Responses to Nitrogen Dose and Form in Chinese Fir Plantations in Southern China

作   者：Caixia Liu, Yuhong Dong, Lingyu Hou, Nan Deng, Ruzhen Jiao
期刊名称：Curr Microbiol
影响因子：1.322
卷 期 号：74
页     码：396-403
关键词：Acidobacteria · Subgroup · Ammonium · Nitrate · N dose · Forest soil
论文摘要：
Acidobacteria is a new bacterial group, identifed by molecular research, which is widely distributed and has specifc ecological functions in forest soil. In this study, we investigated Acidobacteria response to N input, and the effects were related to N form and dose. The experimental design included two N forms (NH4+-N and NO3−-N) and fve levels of N deposition (0, 20, 40, 60, 80 kg N ha−1) for 2 years. Research into the Acidobacteria community was conducted using 16Sr RNA gene-based high-throughput pyrosequencing methods. Acidobacteria OTUs and N had a negative relationship in 0–60 kg ha−1 year−1; however, at N doses beyond a certain size, nitrogen might promote an increase in Acidobacteria OTUs. The Acidobacteria relative abundance under NH 4 +-N treatment was higher than under NO 3 −-N treatment. Acidobacteria relative abundance decreased with increasing of NH4+-N dose, but increased with increasing NO3−-N dose. Overall, 13 different Acidobacteria subgroups were identifed, with Gp1, Gp2, and Gp3 being dominant. Signifcant differences in Acidobacteria distribution were primarily caused by N input and pH value. The environmental factors of N were all negatively related to Acidobacteria distribution in low N dose treatments (0–20 kg ha−1 year−1), but were positively related in response to N dose treatments (40–80 kg ha−1 year−1).