Comprehensive analysis of differentially expressed genes reveals the molecular response to elevated CO2 levels in two sea buckthorn cultivars

作   者：Guoyun Zhanga, Tong Zhang, Juanjuan Liu, Jianguo Zhang, Caiyun He
期刊名称：Gene
影响因子：2.498
卷 期 号：
页     码：120-127
关键词：Sea buckthorn CO2 concentration Transcriptome Differentially expressed genes Molecular mechanism
论文摘要：
Atmospheric carbon dioxide (CO2) concentration increases every year. It is critical to understand the elevated
CO2 response molecular mechanisms of plants using genomic techniques. Hippophae rhamnoides L. is a high stress
resistance plant species widely distributed in Europe and Asia. However, the molecular mechanism of elevated
CO2 response in H. rhamnoides has been limited. In this study, transcriptomic analysis of two sea buckthorn
cultivars under different CO2 concentrations was performed, based on the next-generation illumina sequencing
platform and de novo assembly. We identified 4740 differentially expressed genes in sea buckthorn response to
elevated CO2 concentrations. According to the gene ontology (GO) results, photosystem I, photosynthesis and
chloroplast thylakoid membrane were the main enriched terms in ‘xiangyang’ sea buckthorn. In ‘zhongguo’ sea
buckthorn, photosynthesis was also the main significantly enriched term. However, the number of photosynthesis
related differentially expressed genes were different between two sea buckthorn cultivars. Our GO and
pathway analyses indicated that the expression levels of the transcription factors WRKY, MYB and NAC were
significantly different between the two sea buckthorn cultivars. This study provides a reliable transcriptome
sequence resource and is a valuable resource for genetic and genomic researches for plants under high CO2
concentration in the future.