Consistency between molecular phylogeny and morphological classification of the Salix matsudana Koidz. complex (Salicaceae)

作   者：王兆山，杜淑辉，李云晓，王东升，张建国
期刊名称：Genetics and Molecular Research
影响因子：0.775
卷 期 号：14(3)
页     码：8663-8671
关键词：旱柳 杨柳科
论文摘要：
The morphological species concept is based on morpho-logical traits, which are often subject to subjectivity or artifact. Molecular evidence is needed to test the reliability of morphological classification of taxa that are controversial and to provide appropriate taxonomic de-limitation. In this study, we used 15 single-copy nuclear loci and 2 chlo-roplast fragments to verify the morphological classification of the Salix matsudana Koidz. complex using phylogenetic approaches. Complete sequence alignment showed slight diversification in nuclear sequences and no variety in chloroplast DNA fragments. Phylogenetic trees revealed a monophyletic group consisting of all individuals of S. matsudana and 2 clades within this group, with a 100% bootstrap support value and 1.00 posterior probability. The topology of the phylogenetic trees was highly consistent with the morphological classification of the S. matsudana com-plex. Verifying the genetic background of these classification units based on remarkable morphological differences will provide a foundation for future studies of Salix and the breeding of new horticultural varieties.