Development and characterization of polymorphic Genic-SSR markers in larix kaempferi

作   者：陈兴斌；谢允慧；孙晓梅
期刊名称：Molecules
影响因子：2.416
卷 期 号：20
页     码：6060-6067
关键词：Larix kaempferi; unigene sequences; simple sequence repeat (SSR);
论文摘要：
New simple sequence repeat (SSR) markers were developed in the Japanese
larch (Larix kaempferi) using unigene sequences for further genetic diversity studies and
the genetic improvement of breeding programs. One thousand two handred and thirty five
(1235) primer pairs were tested and 165 successfully identified in L. kaempferi. Out of the
amplified candidate markers, 145 (90.6%) exhibited polymorphism among 24 individuals
of L. kaempferi, with the number of alleles per locus (Na), observed heterozygosity (Ho),
expected heterozygosity (He) and polymorphic information content (PIC) averaging at
4.510, 0.487, 0.518 and 0.459, respectively. Cross-species amplification of randomly
selection of 30 genic-SSRs among the 145 polymorphic ones showed that 80.0% of
the SSRs could be amplified in Larix olgensis, 86.7% could be amplified in
Larix principi-rupprechtii and 83.0% could be amplified in Larix gmelinii. High rates of
cross-species amplification were observed. The genic-SSRs developed herein would be a
valuable resource for genetic analysis of Larix kaempferi and related species, and also have
the potential to facilitate the genetic improvement and breeding of larch.