Identification of microRNAs Involved in Regeneration of the Secondary Vascular System in Populus tomentosa Carr.

作   者：唐芳, HairongWei, 赵树, 王丽娟, HuanquanZheng， 卢孟柱
期刊名称：Frontiers in Plant Science
影响因子：4.495
卷 期 号：7
页     码：724
关键词：
论文摘要：
Wood formation is a complex developmental process governed primarily by a regulatory transcription network. MicroRNAs can exert regulatory roles over transcription of their target genes involved in plant growth and development through mRNA degradation and translational repression. In this study, we used the regeneration of the secondary vascular system established in Populus tomentosa to harvest tissues generated from differentiating xylem in a time series for small RNA high-throughput sequencing. The sequencing data were analyzed led to the identification of 209 known and 187 novel miRNAs during this regeneration process. Degradome sequencing analysis was then performed and 157 and 75 genes targeted by 21 known and 30 novel miRNA families were obtained respectively. GO enrichment of these target genes revealed that the targets of 15 miRNAs were enriched in the auxin signaling pathway, cell differentiation, meristem development and pattern specification process. They were major biological events during the regeneration of secondary vascular system including vascular cambium initiation, formation, and differentiation stages in sequence. This study provides the basis for further analysis of these miRNAs to gain a better insight into their regulatory roles in wood development in trees.