The populus trichocarpa pthsp17.8 involved in heat and salt stress tolerances

作   者：李建波， 张进， 贾会霞， 李煜， 徐向东，王丽娟，卢孟柱*
期刊名称：Plant Cell Reports
影响因子：3.088
卷 期 号：35
页     码：1587-1599
关键词：
论文摘要：
Small heat shock proteins (sHSPs) play important
roles in response to diverse biotic and abiotic stresses,
especially in heat tolerance. However, limited information
is available on the stress tolerance roles of sHSPs in woody
species. To explore the function of sHSPs in poplar, we
isolated and characterized PtHSP17.8 from Populus trichocarpa.
Phylogenetic analysis and subcellular localization
revealed that PtHSP17.8 was a cytosolic class I sHSP.
The gene expression profile of PtHSP17.8 in various tissues
showed that it was significantly accumulated in stem
and root, which was consistent with the GUS expression
pattern driven by promoter of PtHSP17.8. The expression
of PtHSP17.8 could be induced by various abiotic stresses
and significantly activated by heat stress. Overexpression
of PtHSP17.8 enhanced the tolerance to heat and salt
stresses in Arabidopsis. The seedling survival rate, root
length, relative water content, antioxidative enzyme
activities, proline, and soluble sugar content were increased
in transgenic Arabidopsis under heat and salt stresses, but
not in normal condition. The co-expression network of
PtHSP17.8 were constructed and demonstrated many stress
responsive genes included. The stress-related genes in the
co-expression network were up-regulated in the PtHSP17.8
overexpression seedlings. These results suggest that
PtHSP17.8 confers heat and salt tolerances in plants.