A cDNA microarray analysis of the molecular control of poplar wood properties

作   者：Qinjun Huang，Changjun Ding，Weixi Zhang，Bingyu Zhang，Yanguang Chu，Dahai Wang，Xiaohua Su
期刊名称：Journal of Forestry Research
影响因子：0.667
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关键词：Populus deltoides cDNA microarray Expression profile Wood density Microfibrillar angle
论文摘要：
Molecular biological research into wood development
and formation has been the focus in recent years,
but the pace of discovery of related genes and their functions
in the control of wood properties has been slow. The
microarray technique—with its advantages of high
throughput capacity, sensitivity, and reliability over other
tools developed for investigating genes expression patterns—is
capable of rapidly assaying thousands of genes.
In this study, a cDNA microarray prepared from two cDNA
libraries of developing poplar xylem tissues was used to
assay gene expression patterns in immature xylem tissues
at different heights from the main stem of Populus deltoides
(15 years old), which was confirmed to have distinct
wood properties (microfibrillar angle, woody density) by
X-ray. Two hundred seventy-four transcripts with differential
expression profiles between the chips were screened
out, and the individual clones were subjected to 50
sequencing. Using bioinformatic analysis, we identified candidate genes that may influence poplar wood properties,
many of which belong to various regulatory and signal
transduction gene families, such as zinc finger protein
transcription factor, DNA-binding transcription factor,
ethylene response factors, and so on. The results suggest
that these genes may regulate enzymes involved in wood
formation. Further work will be performed to clone these
genes and determine how they influence poplar wood
properties.