Physiological, biochemical, and proteome profiling reveals key pathways underlying the drought-stress responses of Hippophae rhamnoides

作   者：CaiYun He, GuoYun Zhang, JianGuo Zhang*, AaiGuo Duan, HongMei Luo.
期刊名称：Proteomics
影响因子：4.079
卷 期 号：16
页     码：2688-2697
关键词：DIGE / Drought stress / Epigenetic modification / Hippophae rhamnoides / Plant proteomics / Proteome profiling
论文摘要：
The effects of drought on plant growth and development are occurring as a result of climate
change and the growing scarcity of water resources. Hippophae rhamnoides has been exploited
for soil and water conservation for many years. However, the outstanding drought-resistance
mechanisms possessed by this species remain unclear. The protein, physiological, and biochemical
responses to medium and severe drought stresses in H. rhamnoides seedlings are
analyzed. Linear decreases in photosynthesis rate, transpiration rate, and the content of indole
acetic acid in roots, as well as a linear increase in the contents of abscisic acid, superoxide
dismutase, glutathione reductase, and zeatin riboside in leaves are observed as water potential
decreased. At the same time, cell membrane permeability, malondialdehyde, stomatal conductance,
water use efficiency, and contents of zeatin riboside in roots and indole acetic acid
in leaves showed nonconsistent changes. DIGE and MS/MS analysis identified 51 differently
expressed protein spots in leaves with functions related to epigenetic modification and PTM
in addition to normal metabolism, photosynthesis, signal transduction, antioxidative systems,
and responses to stimuli. This study provides new insights into the responses and adaptations
in this drought-resistant species and may benefit future agricultural production.