The conserved salt-responsive genes in the roots of Populus canescens and Arabidopsis thaliana

作   者：Jie Luo,Wenguang Shia, Hong Lic, Dennis Janz, Zhi-Bin Luo
期刊名称：Environmental and Experimental Botany
影响因子：3.712
卷 期 号：129
页     码：48-56
关键词：Transcriptome,Salinity,Populus,Co-expression,Hub genescis-Elements
论文摘要：
To dissect the salt responsive genes in the roots of Populus canescens after salt exposure and to
characterize the conserved transcripts with differential expression in the roots of P. canescens and
Arabidopsis thaliana under salinity, P. canescens grown in sandy soil was exposed to 150 mM NaCl for 18
days and the transcriptional profiling was analyzed in the roots by using a whole genome poplar array
chip. The raw data of the Affymetrix Arabidopsis genome arrays were obtained from the online array data
bank and statistically analysed as that of the poplar. In the roots of P. canescens exposed to salinity,
about 860 genes displayed significantly differential expression, including 647 up-regulated and 213
down-expressed genes. In the roots of A. thaliana, 1292 genes were up- and 718 down-expressed. Among
the differentially expressed genes between P. canescens and A. thaliana, a set of common genes (128
genes) showed the same change pattern in response to salinity, including 114 induced and 14 repressed
genes. A salt-responsive co-expression network was constructed with 98 common genes. Among the
co-expressed genes, 22 genes were defined as hub genes which were involved in fundamental biological
processes such as abiotic stimulus and signal transduction. Moreover, the cis-regulatory elements were
found in the conserved motifs of hub genes. These results suggest that P. canescens and A. thaliana
possess conserved salt-responsive genes and that cis-elements in the conserved motifs of hub genes play
a crucial role in coordinating the co-expression of the common genes underlying the physiological
acclimation to salinity in the roots of P. canescens and A. thaliana.