Development of chloroplast genomic resources for Akebia quinata
作 者：Bin Li,Yanda Li,Qifei Cai,Furong Lin,Ping Huang,Yongqi Zheng
期刊名称：Conservation Genet Resour
影响因子：0.446
卷 期 号： (2016) 8:
页 码：447–449
关键词：
论文摘要：
Abstract Akebia quinata is an over-harvesting shrub
which faces an urgent need for reasonable conservation
strategies. In this study, we report its complete chloroplast
genome by Illumina pair-end sequencing. The total cp
genome size was 157,817 bp in length, containing a pair of
inverted repeats of 26,143 bp, separated by large single
copy and small single copy of 86,543 bp and 18,988 bp,
respectively. The chloroplast genome of A. quinata
encodes 113 different genes, including 79 protein-coding
genes, 30 transfer RNAs and 4 ribosomal RNAs. A total of
47 perfect cp microsatellites were analyzed in the A. qui-
nata. The majority of the SSRs in this cp genome are
mononucleotides (74.47 %). Regions of the highest vari-
ability were sought out by comparing with A. trifoliate.
There are only 385 nucleotide substitutions and 141 indels
between the two genomes. Six highly variable regions were
identified including four intergenic regions and two coding
regions.