Molecular identification and genetic variation of varieties of Styphnolobium japonicum (Fabaceae) using SRAP markers

作   者：R.X. Sun*, C.H. Zhang*, Y.Q. Zheng, Y.C. Zong, X.D. Yu and P. Huang
期刊名称：Genetics and Molecular Research
影响因子：0.764
卷 期 号：
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关键词：Styphnolobium japonicum; SRAP; Molecular identification; Genetic variation; DNA fingerprints
论文摘要：
ABSTRACT. Thirty-four Styphnolobium japonicum varieties were
analyzed using sequence-related amplified polymorphism (SRAP)
markers, to investigate genetic variation and test the effectiveness of
SRAP markers in DNA fingerprint establishment. Twelve primer pairs
were selected from 120 primer combinations for their reproducibility
and high polymorphism. We found a total of 430 amplified fragments,
of which 415 fragments were considered polymorphic with an average
of 34.58 polymorphic fragments for each primer combination. The
percentage of polymorphic fragments was 96.60%, and four primer
pairs showed 100% polymorphism. Moreover, simple matched
coefficients ranged between 0.68 and 0.89, with an average of 0.785,
indicating that the genetic variation among varieties was relatively
low. This could be because of the narrow genetic basis of the selected
breeding material. Based on the similarity coefficient value of 0.76, the
varieties were divided into four major groups. In addition, abundant
and clear SRAP fingerprints were obtained and could be used toestablish DNA fingerprints. In the DNA fingerprints, each variety had
its unique pattern that could be easily distinguished from others. The
results demonstrated that 34 varieties of S. japonicum had a relatively
narrow genetic variation. Hence, a broadening of the genetic basis of
breeding material is necessary. We conclude that establishment of DNA
fingerprint is feasible by means of SRAP markers.