Metabolomics reveals constitutive metabolites that contribute resistance to fall webworm (Hyphantria cunea) in Populus deltoides

作   者：王丽娟，曲良建，胡建军，张立伟，唐芳，卢孟柱
期刊名称：Environmental and Experimental Botany
影响因子：4.369
卷 期 号：136
页     码：31-40
关键词：woody plants; poplar; insect; Hyphantria cunea; resistance; plant metabolomics
论文摘要：
Abstract
Constitutive chemical defenses play important roles in protecting trees against insects.
Studying the constitutive chemical resistance of trees to insect would thus help control
the pests and minimize the associated ecological and economic losses. Poplar is an
ecologically and economically important tree species but has been greatly damaged
by fall webworm (Hyphantria cunea) in China. Here we investigated H. cunea
resistance in 7 poplar cultivars commonly planted in China by applying bioassays,
and analyzed metabolic profiles of H. cunea -resistant and -susceptible cultivars.
Substantial variation in chemical profiles between resistant and susceptible genotypes
was detected. Ninety-two distinguishing metabolites were identified. Correlation
analysis indicated that hydroxycinnamates, benzenoids and their derivatives
significantly positively correlated with resistance against H. cunea, whereas sucrose,
glutamic acid and myo-inositol showed negative correlations. Bioassays of H. cunea
given an artificial diet supplemented with these compounds confirmed their effects
but indicated that they each have different roles. Metabolite enrichment and gene
expression analysis showed that phenylpropanoid biosynthesis in poplar is important
for constitutive resistance against H. cunea. Induced experiment found that some of
the constitutive resistance-related metabolites were induced by H. cunea indicating
that these metabolites also play a role in induced resistance. This study provides
further insight into the molecular mechanisms behind H. cunea resistance in poplar
and suggests candidate metabolites that may serve as biomarkers for breeding of
resistant poplar cultivars.