An Empirical Assessment of Transgene Flow from a Bt Transgenic Poplar Plantation

作   者：胡建军，张进，陈香玲，吕金慧，贾会霞，赵树堂，卢孟柱
期刊名称：PLOS ONE
影响因子：2.806
卷 期 号：12(1)
页     码：e0170201
关键词：
论文摘要：
To assess the possible impact of transgenic poplar plantations on the ecosystem, we analyzed
the frequency and distance of gene flow from a mature male transgenic Populus nigra
plantation carrying the Bacillus thuringiensis toxin gene (Bt poplar) and the survival of Bt
poplar seeds. The resultant Bt poplar seeds occurred at a frequency of ~0.15% at 0 m to
~0.02% at 500 m from the Bt poplar plantation. The germination of Bt poplar seeds diminished
within three weeks in the field (germination rate from 68% to 0%) compared to 48%
after three weeks of storage at 4˚C. The survival rate of seedlings in the field was 0% without
any treatment but increased to 1.7% under the addition of four treatments (cleaning and
trimming, watering, weeding, and covering with plastic film to maintain moisture) after being
seeded in the field for eight weeks. The results of this study indicate that gene flow originating
from the Bt poplar plantation occurred at an extremely low level through pollen or seeds
under natural conditions. This study provides first-hand field data on the extent of transgene
flow in poplar plantations and offers guidance for the risk assessment of transgenic poplar
plantations.