Overexpression of a peroxiredoxin gene from Tamarix hispida, ThPrx1, confers tolerance to oxidative stress in yeast and Arabidopsis

作   者：王留强，李真，王超，王德印，王玉成，卢孟柱
期刊名称：Journal of Plant Biology
影响因子：1.437
卷 期 号：60(6)
页     码：548-557
关键词：Oxidative stress, Peroxiredoxin, Reactive oxygen species, Tamarix hispida
论文摘要：
Peroxiredoxins (Prxs) are ubiquitous thiol-specific
antioxidant enzymes that are critically involved in cell defense
and protect cells from oxidative damage. In this study, a
putative Type II Prx (ThPrx1) was identified and characterized
from Tamarix hispida. The expression of ThPrx1 is highly
induced in response to hydrogen peroxide (H2O2) and methyl
viologen (MV) stresses. When expressed ectopically, ThPrx1
showed enhanced tolerance against oxidative stress in yeast
and Arabidopsis. In addition, transgenic Arabidopsis plants
overexpressing ThPrx1 displayed improved seedling survival
rates and increased root growth and fresh weight gain under
H2O2 and MV treatments. Moreover, transgenic Arabidopsis
plants showed decreased accumulation of H2O2, superoxide
(O2
•−
) and malondialdehyde (MDA), increased superoxide
dismutase (SOD) activity compared to wild-type (WT) plants
under oxidative stress. Moreover, transgenic plants maintained
higher photosynthesis efficiency and lower electrolyte leakage
rates than that of WT plants under stress conditions. These
results clearly indicated that ThPrx1 plays an important role
in cellular redox homeostasis under stress conditions, leading
to the maintenance of membrane integrity and increased
tolerance to oxidative stress.