Characterization of a plant-transformation-ready large-insert BIBAC library of Populus euphratica Oliv.

作   者：11. Jing Zhou, Xin Liu, Shu-Tang Zhao, Jian-Jun Hu, Jie-Wei Zhang, Jie-Hua Wang, Xiao-Peng Peng, Xiao-Li Qi, Tie-Long Cheng, Meng-Zhu Lu
期刊名称：Plant Molecular Biology
影响因子：3.540
卷 期 号：DOI: 10.1007/s11103-018-0755-4
页     码：
关键词： transgenomics, Populus euphratica, salt tolerance, functional genomics, Arabidopsis thaliana
论文摘要：
Key message Transgenomics for gene discovery in P. euphratica.
Transgenomics, a member of the omics family of methodologies, is characterized as
the introduction of DNA from one organism into another on a genome-wide scale
followed by the identification of recipients with altered phenotypes. This strategy allows
investigators to identify the gene(s) involved in these phenotypic changes. It is
particularly promising for woody plants that have a long life cycle and for which
molecular tools are limited. In this study, we constructed a large-insert binary bacterial
artificial chromosome library of Populus euphratica, a stress-tolerant poplar species,
which included 55,296 clones with average insert sizes of about 127 kb. To date, 1077
of the clones have been transformed into Arabidopsis thaliana via Agrobacterium by
the floral dip method. Of these, 69 transgenic lines showed phenotypic changes
represented by diverse aspects of plant form and development, 22 of which werereproducibly associated with the same phenotypic change. One of the clones
conferring transgenic plants with increased salt tolerance, 002A1F06, was further
analyzed and the 127,284 bp insert in this clone harbored eight genes that have been
previously reported to be involved in stress resistance. This study demonstrates that
transgenomics is useful in the study of functional genomics to identification of novel
gene (s) responsible for economically important traits. Thus, transgenomics can also
be used for validation of quantitative trait loci mapped by molecular markers.