Proteome-level analysis of metabolism- and stress-related proteins during seed dormancy and germination in Gnetum parvifolium.

作   者：Chang Ermei, Deng Nan, Jin Zhang, Liu Jianfeng, Chen Lanzhen, Zhao Xiulian, M. Abbas, Jiang Zeping*, Shi Shengqing*
期刊名称：Journal of agricultural and food chemistry
影响因子：3.412
卷 期 号：66(11)
页     码：3019-3029
关键词：是
论文摘要：
ABSTRACT: Gnetum parvifolium is a rich source of materials for traditional medicines, food, and oil, but little is known about
the mechanism underlying its seed dormancy and germination. In this study, we analyzed the proteome-level changes in its seeds
during germination using isobaric tags for relative and absolute quantitation. In total, 1,040 differentially expressed proteins were
identified, and cluster analysis revealed the distinct time points during which signal transduction and oxidation?reduction activity
changed. Gene Ontology analysis showed that “carbohydrate metabolic process” and “response to oxidative stress” were the main
enriched terms. Proteins associated with starch degradation and antioxidant enzymes were important for dormancy-release, while
proteins associated with energy metabolism and protein synthesis were up-regulated during germination. Moreover, proteininteraction
networks were mainly associated with heat-shock proteins. Furthermore, in accord with changes in the energy
metabolism- and antioxidant-related proteins, indole-3-acetic acid, Peroxidase, and soluble sugar content increased, and the starch
content decreased in almost all six stages of dormancy and germination analyzed (S1?S6). The activity of superoxide dismutase,
abscisic acid, and malondialdehyde content increased in the dormancy stages (S1?S3) and then decreased in the germination
stages (S4?S6). Our results provide new insights into G. parvifolium seed dormancy and germination at the proteome and
physiological levels, with implications for improving seed propagation.
KEYWORDS: Gnetum parvifolium, proteome, seed germination, seed dormancy, iTRAQ