Comparative Metabolomic Analysis of the Cambium Tissue of Non-transgenic and Multi-Gene Transgenic Poplar (Populus euramericana ‘Guariento’)

作   者：Kun Ning，Changjun Ding，Wenxu Zhu，Weixi Zhang，Yufeng Dong，Yingbai Shen and Xiaohua Su*
期刊名称：Frontiers in plant science
影响因子：3.677
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关键词：metabolomics, multi-gene transgenic poplar, cambium, GC–MS, UPLC–MS/MS
论文摘要：
Poplar, a model for woody plant research, is the most widely distributed tree species in
the world. Metabolites are the basis of phenotypes, allowing an intuitive and effective
understanding of biological processes and their mechanisms. However, metabolites
in non-transgenic and multi-gene transgenic poplar remains poorly characterized,
especially in regards of the influences on quantity and in the analysis of the relative
abundance of metabolites after the introduction of multi stress-related genes. In this
study, we investigated the cambium metabolomes of one non-transgenic (D5-0) and two
multi-gene (vgb, SacB, JERF36, BtCry3A, and OC-I) transgenic lines (D5-20 and D5-21)
of hybrid poplar (Populus euramericana ‘Guariento’) using both gas chromatography–
mass spectrometry (GC–MS) and ultra-performance liquid chromatography-tandem
mass spectrometry (UPLC–MS/MS). We aimed to explore the effects of the exogenous
genes on metabolite composition and to screen out metabolites with important
biological functions. Finally, we identified 239 named metabolites and determined
their relative abundance. Among these, 197 metabolites had a different abundance
across the three lines. These methabolites spanned nine primary and 44 secondary
metabolism pathways. Arginine and glutamate, as substrates and intermediates in
nitrogen metabolism, and important in growth and stress-related processes, as well
as sucrose, uridine diphosphate glucose, and their derivatives, precursors in cell wall
pathways, and catechol, relevant to insect resistance, differed greatly between the
genetically modified and non-transgenic poplar. These findings may provide a basis for
further study of cambium metabolism, and fully understand metabolites associated with
stress response.