Genome Comparison Reveals Mutation Hotspots in the Chloroplast Genome and Phylogenetic Relationships of Ormosia Species

作   者：HongshanLiu,1,2 ZhihaiSu,2 ShuiqingYu,2 JialinLiu,2 XiaojuanYin,2 GuoweiZhang,2 WeiLiu,2 andBinLi
期刊名称：BioMed Research International
影响因子：2.197
卷 期 号：2019
页     码：1-11
关键词：
论文摘要：
ThepapilionoidlegumegenusOrmosiacomprisesapproximately130species,whicharedistributedmostlyintheNeotropics,with somespeciesineasternAsiaandnortheasternAustralia.Thetaxonomyandevolutionaryhistoryremainunclearduetothelackof arobustspecies-levelphylogeny.Chloroplastgenomescanprovideimportantinformationforphylogeneticandpopulationgenetic studies.Inthisstudy,wedeterminedthecompletechloroplastgenomesequencesoffiveOrmosiaspeciesbyIlluminasequencing. TheOrmosiachloroplastgenomesdisplayedthetypicalquadripartitestructureofangiosperms,whichconsistedofapairofinverted regionsseparatedbyalargesingle-copyregionandasmallsingle-copyregion.Thelocationanddistributionofrepeatsequencesand microsatellitesweredetermined.Comparativeanalyseshighlightedawidespectrumofvariation,withtrnK-rbcL,atpE-trnS-rps4, trnC-petN, trnS-psbZ-trnG, trnP-psaJ-rpl33, and clpP intron being the most variable regions. Phylogenetic analysis revealed that Ormosia isinthePapilionoideae cladeandissistertothe Lupinus clade.Overall,thisstudy,whichprovidesOrmosia chloroplast genomic resourcesanda comparative analysis of Ormosia chloroplast genomes,will be beneficialfor theevolutionarystudy and phylogenetic reconstructionof the genusOrmosia and molecular barcoding inpopulation genetics andwill provide insight into thechloroplastgenomeevolutionoflegumes.