张立峰

科研项目

（1）科技创新2030-“农业生物育种”重大项目子课题，“落叶松抗旱多基因聚合新种质创制及新材料评价”，2023.09-2025.12，主持，经费238.02万元。

（2）国家自然科学基金面上项目，“落叶松miR396-GRF影响体细胞胚发育进程的分子调控机理”，2022.01-2025.12，主持，经费75.40万元。

（3）中国林科院林业所中央级公益性科研院所基本科研业务费专项资金，“落叶松LaTCTP启动子功能研究及瞬时表达体系的建立”，2019.01-2020.12，主持，经费25.00万元。

（4）国家自然科学基金青年科学基金项目，“落叶松miR156b调控体细胞胚发育的功能解析”，2017.01-2019.12，主持，经费23.84万元。

主要成果

授权发明专利

（1）张立峰，党少飞，齐力旺. 一种落叶松LaTCTP启动子及其应用:ZL202211016162.5 [P]. 2025-10-31.

（2）DANG Shaofei，ZHANG Lifeng，QI Liwang. Method For Inducing and Rapidly Proliferating Callus of Lycium Barbarum L.:LU502067[P]. 2023-11-15.

（3）张立峰，韩素英，齐力旺，李水根，徐海燕. 一种落叶松胚性细胞的遗传转化方法：ZL201510953701.1[P]. 2020-04-21.

（4）张立峰，韩素英，齐力旺，李水根，张俊红. 一种从基因组DNA中获得侧翼序列的方法: ZL201310452575.2[P]. 2017-12-21.

（5）张守攻，李水根，齐力旺，张立峰，韩素英，朱彩虹. 落叶松miR166a在调控植物发育中的应用: ZL201310452485.3[P]. 2017-08-15.

生物育种新种质中间试验行政许可：

（1）转CMO-BADH基因日本落叶松2个株系中间试验行政许可（林技许准字〔2026〕2号）

（2）转RUBY基因日本落叶松1个株系中间试验行政许可（林技许准字〔2026〕3号）

（3）转RUBY-HYG基因日本落叶松1个株系中间试验行政许可（林技许准字〔2026〕4号）

代表性文章

（1）Yuqin Huang, Ruxin Zhang, Xin Li, Liwang Qi, Lifeng Zhang*. Genome-wide identification of GRF and GIF genes in Larix kaempferi and their potential association with somatic embryogenesis, hormone responses, and miR396-mediated regulation[J]. Plant Cell Tiss Organ Cult, 2026, 165:77.

（2）Xin Li, Yuqin Huang, Wenhua Yang, Liwang Qi, Lifeng Zhang*, Chenghao Li*. Conserved miR156 Mediates Phase-Specific Coordination Between Cotyledon Morphogenesis and Embryo Dormancy During Somatic Embryogenesis in Larix kaempferi[J]. International Journal of Molecular Sciences, 2025, 26(17):8206.

（3）Xin Li, Junchen Wang, Yuqin Huang, Chenghao Li, Liwang Qi, Lifeng Zhang*. SPL gene family in Larix kaempferi: identification, functional analysis, and roles in somatic embryogenesis and hormonal regulation[J]. Trees, 2025, 39:95.

（4）黄雨芹, 张如鑫, 张馨元, 王俊臣, 齐力旺, 张立峰*. 落叶松LkTCTP启动子的克隆及表达分析[J]. 林业科学研究, 2025, 38(2): 48-57.

（5）Junchen Wang^(#), Lifeng Zhang^(#), Liwang Qi, Shougong Zhang*. Integrated transcriptomic and metabolic analyses provide insights into the maintenance of embryogenic potential and the biosynthesis of phenolic acids and flavonoids involving transcription factors in Larix kaempferi (Lamb.) Carr[J]. Frontiers in Plant Science, 2022, 13:1056930.

（6）Shaofei Dang^(#), Lifeng Zhang^(#)*, Suying Han, Liwang Qi. Agrobacterium-Mediated Genetic Transformation of Larix kaempferi (Lamb.) Carr. Embryogenic Cell Suspension Cultures and Expression Analysis of Exogenous Genes [J]. Forests, 2022, 13(9), 1436.

（7）Yanru Fan, Wanfeng Li, Zhexin Li, Shaofei Dang, Suying Han, Lifeng Zhang *, Qi Liwang*. Examination of the Transcriptional Response to LaMIR166a Overexpression in Larix kaempferi (Lamb.) Carr[J]. Biology-BASEL, 2021, 10(7), 576.

（8）Lifeng Zhang, Yanru Fan, Qian Lan, Liwang Qi, Suying Han. Expression of the SPL-like gene LaSPL9 in Japanese larch (Larix leptolepis) is regulated by miR156 during somatic embryogenesis[J]. Trees, 2021, 35:1727-1737.

（9）范艳如, 兰倩, 韩素英, 齐力旺, 张立峰*. 日本落叶松LaSPL2和LaSPL3在体细胞胚发育中的表达分析[J]. 林业科学研究, 2021, 34(5):80-88.

（10）Lifeng Zhang, Qian Lan, Suying Han, Liwang Qi. A GH3-like gene, LaGH3, isolated from hybrid larch (Larix leptolepis×Larix olgensis) is regulated by auxin and abscisic acid during somatic embryogenesis[J]. Trees, 2019, 33(12):1723–1732.

（11）Zhexin Li^(#), Shuigen Li^(#), Lifeng Zhang^(#), Suying Han, Wanfeng Li, Haiyan Xu, Wenhua Yang, Yali Liu, Yanru Fan, Liwang Qi. Over-expression of miR166a inhibits cotyledon formation in somatic embryos and promotes lateral root development in seedlings of Larix leptolepis[J]. Plant Cell Tiss Organ Cult, 2016,127:461-473.

（12）Li-feng Zhang, Wan-feng Li, Hai-yan Xu, Li-wang Qi, Su-ying Han. Cloning and characterization of four differentially expressed cDNA encoding NFYA homologous involved in response to ABA during somatic embryogenesis in Japanese larch (Larix leptolepis) [J]. Plant Cell Tiss Organ Cult, 2014, 117(2): 293-304.

（13）Li-feng Zhang, Wan-feng Li, Su-ying Han, Wen-hua Yang, Li-wang Qi. cDNA cloning, genomic organization and expression analysis during somatic embryogenesis of the translationally controlled tumor protein (TCTP) gene from Japanese larch (Larix leptolepis) [J]. Gene, 2013, 529(1): 150-158.

（14）张立峰, 李水根, 韩素英, 张守攻, 齐力旺. 落叶松转基因育种体系的建立及其产业化应用[J]. 生物产业技术, 2013, 03: 7-11